Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Identifieur interne : 005171 ( Main/Exploration ); précédent : 005170; suivant : 005172Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Auteurs : Shannon L. Emery [États-Unis] ; Dean D. Erdman [États-Unis] ; Michael D. Bowen [États-Unis] ; Bruce R. Newton [États-Unis] ; Jonas M. Winchell [États-Unis] ; Richard F. Meyer [États-Unis] ; Suxiang Tong [États-Unis] ; Byron T. Cook [États-Unis] ; Brian P. Holloway [États-Unis] ; Karen A. Mccaustland [États-Unis] ; Paul A. Rota [États-Unis] ; Bettina Bankamp [États-Unis] ; Luis E. Lowe [États-Unis] ; Tom G. Ksiazek [États-Unis] ; William J. Bellini [États-Unis] ; Larry J. Anderson [États-Unis]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN viral (génétique), ARN viral (génétique), ARN viral (isolement et purification), Amorces ADN (génétique), Animaux, Cellules Vero, Humains, RT-PCR (), Reproductibilité des résultats, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ADN viral, ARN viral, Amorces ADN, Virus du SRAS.
- isolement et purification : ARN viral, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Humains, RT-PCR, Reproductibilité des résultats, Sensibilité et spécificité, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Chlorocebus aethiops, DNA Primers (genetics), DNA, Viral (genetics), Humans, RNA, Viral (genetics), RNA, Viral (isolation & purification), Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction (statistics & numerical data), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Vero Cells.
- MESH :
- chemical , genetics : DNA Primers, DNA, Viral, RNA, Viral.
- chemical , isolation & purification : RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- statistics & numerical data : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Base Sequence, Chlorocebus aethiops, Humans, Reproducibility of Results, Sensitivity and Specificity, Vero Cells.
Abstract
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
Url:
DOI: 10.3201/eid1002.030759
PubMed: 15030703
PubMed Central: 3322901
Affiliations:
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Le document en format XML
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Emery</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D." last="Erdman">Dean D. Erdman</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bowen, Michael D" sort="Bowen, Michael D" uniqKey="Bowen M" first="Michael D." last="Bowen">Michael D. 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Winchell</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Meyer, Richard F" sort="Meyer, Richard F" uniqKey="Meyer R" first="Richard F." last="Meyer">Richard F. Meyer</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Cook, Byron T" sort="Cook, Byron T" uniqKey="Cook B" first="Byron T." last="Cook">Byron T. Cook</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Holloway, Brian P" sort="Holloway, Brian P" uniqKey="Holloway B" first="Brian P." last="Holloway">Brian P. Holloway</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Mccaustland, Karen A" sort="Mccaustland, Karen A" uniqKey="Mccaustland K" first="Karen A." last="Mccaustland">Karen A. Mccaustland</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bankamp, Bettina" sort="Bankamp, Bettina" uniqKey="Bankamp B" first="Bettina" last="Bankamp">Bettina Bankamp</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lowe, Luis E" sort="Lowe, Luis E" uniqKey="Lowe L" first="Luis E." last="Lowe">Luis E. Lowe</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Ksiazek, Tom G" sort="Ksiazek, Tom G" uniqKey="Ksiazek T" first="Tom G." last="Ksiazek">Tom G. Ksiazek</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><affiliation wicri:level="2"><nlm:aff id="aff1">Centers for Disease Control and Prevention, Atlanta, Georgia, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, Georgia</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>DNA Primers (genetics)</term><term>DNA, Viral (genetics)</term><term>Humans</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (isolation & purification)</term><term>Reproducibility of Results</term><term>Reverse Transcriptase Polymerase Chain Reaction (statistics & numerical data)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>ARN viral (génétique)</term><term>ARN viral (isolement et purification)</term><term>Amorces ADN (génétique)</term><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>RT-PCR ()</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Séquence nucléotidique</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term><term>Amorces ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>RT-PCR</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Emery, Shannon L" sort="Emery, Shannon L" uniqKey="Emery S" first="Shannon L." last="Emery">Shannon L. Emery</name></region><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><name sortKey="Bankamp, Bettina" sort="Bankamp, Bettina" uniqKey="Bankamp B" first="Bettina" last="Bankamp">Bettina Bankamp</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><name sortKey="Bowen, Michael D" sort="Bowen, Michael D" uniqKey="Bowen M" first="Michael D." last="Bowen">Michael D. Bowen</name><name sortKey="Cook, Byron T" sort="Cook, Byron T" uniqKey="Cook B" first="Byron T." last="Cook">Byron T. Cook</name><name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D." last="Erdman">Dean D. Erdman</name><name sortKey="Holloway, Brian P" sort="Holloway, Brian P" uniqKey="Holloway B" first="Brian P." last="Holloway">Brian P. Holloway</name><name sortKey="Ksiazek, Tom G" sort="Ksiazek, Tom G" uniqKey="Ksiazek T" first="Tom G." last="Ksiazek">Tom G. Ksiazek</name><name sortKey="Lowe, Luis E" sort="Lowe, Luis E" uniqKey="Lowe L" first="Luis E." last="Lowe">Luis E. Lowe</name><name sortKey="Mccaustland, Karen A" sort="Mccaustland, Karen A" uniqKey="Mccaustland K" first="Karen A." last="Mccaustland">Karen A. Mccaustland</name><name sortKey="Meyer, Richard F" sort="Meyer, Richard F" uniqKey="Meyer R" first="Richard F." last="Meyer">Richard F. Meyer</name><name sortKey="Newton, Bruce R" sort="Newton, Bruce R" uniqKey="Newton B" first="Bruce R." last="Newton">Bruce R. Newton</name><name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A." last="Rota">Paul A. Rota</name><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name><name sortKey="Winchell, Jonas M" sort="Winchell, Jonas M" uniqKey="Winchell J" first="Jonas M." last="Winchell">Jonas M. Winchell</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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